大肠杆菌glyA工程菌抗噬菌体菌株选育

摘要

[Objective] This study aimed to select E. Coli strains with phage-resistance. [ Method] Phage-resistant strains of E. Coli glyA genetic engineering bacteria were selected by phage induction and UV-coupling phage induction. [Result] By phage induction, 20 strains with stable resistance were selected from the 24 phage-resistant strains, only one strain showed better growth condition than the original strains, but the enzymatic activities of the 20 strains all were lower than the original strains; 41 phage-resistant strains were selected by UV-coupling phage induction, 39 strains of which had better stability, including 7 strains that showed better growth condition than the original strains and two strains had higher enzymatic activities than the original strains. [ Conclusion ] UV-coupling phage induction is a suitable method to select phage-resistant strains from E. Coli genetic engineering bacteria.%[目的]该研究旨在选育具有噬菌体抗性的大肠杆菌菌株.[方法]利用噬菌体诱发及紫外耦合噬菌体诱发筛选大肠杆菌glyA工程菌的抗噬菌体菌株.[结果]从噬菌体诱发筛选到的24株抗性菌株中筛选出了20株具有稳定抗性的抗性菌株,但其中仅有1株生长势优于原始株,酶活均低于原始株；耦合法诱导得到的41株中有39株具有较好的稳定性,其中7株生长势优于原始株,2株酶活大于原始株.[结论]利用紫外耦合噬菌体诱发筛选大肠杆菌glyA工程菌的抗噬菌体菌株能得到较好的试验效果.