Plasmid containing DNA from a PLASMIDFRAKTION OF B.THURINGIENSIS, E.COLI bacteria strain containing the plasmid, E.COLI genetically engineered bacterial strain, method for preparing the plasmid method of creating a genetically engineered bacterial strain E.COLI, process for producing Bacillus thuringiensis CRYSTAL PROTEINS AND PROCEDURE TO OBTAIN insecticidal activity IN AN ENVIRONMENT

摘要

The crystal protein of Bacillus thuringiensis is an insecticidal substance. Unfortunately growth phase limitations are exhibited by B. thuringiensis in producing this protein. This invention, however, provides a plasmid capable of replication in a bacterial host species and containing expressible heterologous DNA coding for the crystal protein of Bacillus thuringiensis and including an expression mechanism for said heterologous DNA which is recognized by the host species system but does not exhibit substantial growth phase limitations in the bacterial host species. Genetically engineered bacterial host strains transformed by the plasmids of the invention express B. thuringiensis crystal proteins without exhibiting the aforesaid growth phase limitations.