普洱茶渥堆发酵过程中细菌种群的分离与分子鉴定

摘要

[Objective] To isolate, purify and identify bacteria during Pu-erh Tea pile fermentation process. [Method] The bacteria colonization was isolated from Pu-erh Tea in the pile fermentation process and investigated by using molecular biological methods. [Result] The results showed that there are 7 species of bacteria including Bacillus, Klebsiella pneumoniae, Sphingomonas bacteria, Brevibacterium, Rhodococcus, Pseudomonas, Acinetobacter baumannii and so on. The proportion of Bacillus cereus which located in a dominant position is much larger than other bacteria. [ Conclusion ] The study can provide theoretical basis for producing efficient leaven, shortening fermentation time, improving Pu-erh Tea quality, and realizing clean and industrialization production of Pu-erh Tea.%[目的]对普洱茶渥堆发酵过程中的细菌进行分离、纯化和鉴定.[方法]采用传统培养方法与分子生物学方法相结合,对普洱茶渥堆发酵各阶段的细菌进行了分离、纯化及鉴定.[结果]试验表明,普洱茶渥堆发酵各阶段的细菌包括了芽孢杆菌、克雷伯氏菌、鞘氨醇杆菌、短杆菌、红球菌、假单胞菌、鲍曼不动杆菌7个种类,其中芽孢杆菌所占比例远大于其他细菌,处于绝对优势地位.[结论]研究可为研制普洱茶高效发酵剂、缩短发酵时间、改善普洱茶品质,以及实现普洱茶清洁化和产业化生产提供—定的理论依据.