苗药大果木姜子总黄酮与糖类成分含量测定

摘要

[Objective] To establish the method for determination of total flavonoids, total sugar, reducing sugar, and polysaccharide in Cin-namomum migao.[ Method] Using rutin and glucose as control products, the contents of total flavonoids, total sugar, reducing sugar and pol-ysaccharide in C.migao were detected by UV spectrophotometer, and experiments were conducted to investigate whether the method is feasi-ble.[Result] The contents of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao were (8.02 ±0.19) mg/g, (35.26 ±0.96)%, (4.78 ±0.95)%, (30.53 ±0.69)%, respectively.[Conclusion] The method is rapid, simple, accurate, which is a-ble to accomplish the content determination of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao.%[目的]建立测定大果木姜子中总黄酮、总糖、还原糖、多糖含量的方法.[方法]以芦丁和葡萄糖为对照品,采用紫外分光光度计测定大果木姜子中总黄酮、总糖、还原糖、多糖的含量并进行方法学考察.[结果]大果木姜子中总黄酮含量为(8.02±0.19)mg/g,总糖含量为(35.26±0.96)%,还原糖含量为(4.78±0.95)%,多糖含量为(30.53±0.69)%.[结论]该方法快速、简便、准确,可以用于大果木姜子中总黄酮、总糖、还原糖、多糖的含量测定.