疏肝调神针法对创伤后应激障碍模型大鼠静息态脑电信号的影响

摘要

目的:观察疏肝调神针法对创伤后应激障碍(PTSD)模型大鼠静息态脑电活动的影响,为针刺干预PTSD的效应机制研究和临床应用提供依据.方法:将60只Sprague-Dawley (SD)大鼠随机分为空白对照组、模型组、抓取组、帕罗西汀组和针刺组,每组12只.除空白对照组外,其余各组大鼠通过“幽闭+电击”法复制PTSD模型.各组大鼠接受相应的干预后,观察其行为学的改变,并应用多通道脑电采集与分析系统检测其静息态脑电的改变,进行图像分析和统计学处理.结果:与空白对照组比较,模型组大鼠平均逃避潜伏期明显延长(P＜0.05),平台穿越次数、穿越有效区域次数明显减少(P＜0.01);与抓取组比较,帕罗西汀组和针刺组大鼠平均逃避潜伏期明显缩短(均P＜0.05),平台穿越次数、穿越有效区域次数明显增加(均P＜0.05);针刺组平均逃避潜伏期、平台穿越次数及穿越有效区域次数与帕罗西汀组比较,差异均无统计学意义(均P＞0.05).与空白对照组比较,模型组大鼠α波功率谱值明显下降,β波、δ波、(e)波功率谱值明显增高(均P＜0.01);与抓取组比较,帕罗西汀组和针刺组大鼠α波功率谱值明显上升(均P＜0.01),β波、δ波和,波功率谱值明显下降(均P＜0.01);针刺组α波、β波、δ波和3波功率谱值与帕罗西汀组比较,差异均无统计学意义(均P＞0.05).结论:疏肝调神针法可明显改善PTSD大鼠异常脑电活动,可能是疏肝调神针法有效干预PTSD的脑功能机制之一.%Objective:To observe the effect of liver-soothing and mind-regulating acupuncture method on the resting-state electroencephalography (EEG) in rats with post-traumatic stress disorder (PTSD),and to provide evidence for the effect mechanism study and clinical application of acupuncture intervention for PTSD.Methods:Sixty Sprague-Dawley (SD) rats were randomly divided into a blank control group,a model group,a grasping group,a paroxetine group and an acupuncture group,with 12 rats in each group.Except for rats in the blank control group,rats in the other groups were subjected to preparing the PTSD models using 'incarceration plus electric shock' method.After interventions,changes in rat behavior of each group were observed;changes in resting-state EEG were collected and analyzed with multichannel EEG acquisition and analysis system,and image analysis and statistical processing were performed.Results:Compared with the blank control group,the average escape latency in the model group was significantly longer (P＜0.05),and the times of crossing the platform and the effective areas were all significantly reduced (P＜0.01).Compared with the grasping group,the average escape latencies in the paroxetine group and acupuncture group were significantly shortened (P＜0.05),and the times of crossing the platform and the effective areas were all significantly increased (P＜0.05).There were no significant differences in the average escape latency,the times of crossing the platform and the effective areas between the acupuncture group and paroxetine group (all P＞0.05).Compared with the blank control group,the α-wave power spectrum value in the model group was significantly decreased,and the power spectrum values of β-wave,δ-wave and a-wave were significantly increased (all P＜0.01);compared with the grasping group,α-wave power spectrum values in the paroxetine group and acupuncture group were significantly increased (both P＜0.01),and the power spectrum values of β-wave,δ-wave and a-wave were decreased significantly (all P＜0.01).The power spectrum values of α-wave,β-wave,δ-wave and (e)-wave of rats in the acupuncture group were not significantly different from those in the paroxetine group (all P＞0.05).Conclusion:Liver-soothing and mind-regulating acupuncture method can significantly improve the abnormal EEG activity in PTSD rats,which may be one mechanism of liver-soothing and mind-regulating acupuncture method in effectively affecting the brain function in PTSD rats.