逍遥散对慢性束缚应激所致肝郁脾虚证大鼠饥饿素的影响

摘要

目的：观察逍遥散对慢性束缚应激所致肝郁脾虚证大鼠饥饿素（Ghrelin ）的影响，探讨逍遥散的作用机制。方法24只雄性SD大鼠随机分为正常对照组、模型组、逍遥散组，每组8只；正常组不进行任何处理，模型组和逍遥散组通过连续21 d慢性束缚应激建立肝郁脾虚证候模型，逍遥散组于每天上午束缚前灌服逍遥散混悬液3.854 g／kg ，正常组和模型组灌服生理盐水2 mL。比较各组大鼠的一般情况、饮水量及摄食量、体质量变化情况，用ELISA方法测定大鼠血浆及胃底部组织中Ghrelin的浓度。结果束缚21 d后，模型组大鼠体质量、摄食量、饮水量较正常组降低，增加减缓。与模型组相比，逍遥散组体质量、摄食量、饮水量均增加，差异均有统计学意义（ P＜0.05或 P＜0.01）。模型组的血浆及胃底部Ghrelin含量比正常组低（ P＜0.05），而逍遥散组的胃底部Ghrelin含量比模型组高（ P＜0.05），与正常组相近；模型组的血浆Ghrelin含量低于逍遥散组（ P＜0.01）。结论肝郁脾虚证模型大鼠血浆及胃底部组织中Ghrelin含量下降，逍遥散能提高Ghrelin水平，对肝郁脾虚证有明显调节作用，对Ghrelin的调节作用可能是逍遥散治疗肝郁脾虚证的可能机制之一。%Objective This experiment was designed to measure the effect of Xiao Y ao San on treating liver depression and spleen deficiency syndrome by recording the changes of Ghrelin levels in the blood and the stomach bottom of fundic glands in chronic immobilization stress (CIS ) model rats .Methods The 24 male SD rats were randomly divided into 3 groups (8 rats for each group):normal group ,model group and Xiao Yao San group .We didn’t make any deal with normal group ,but model and Xiao Yao San groups by continuous 21 day immobilization stress cause induced liver depression and spleen defi-ciency syndrome ,before every morning bondage administered Xiao Yao San (3 .854 g/Kg・d) to the Xiao Yao San group and administered a saline liquid (2 mL ) to the normal and model groups .We used ELISA to measure the Ghrelin levels in both the blood and stomach bottom after death on day 22 .Result After 21 days ,the model group rat body weight ,food in-take ,water quantity increase slower than normal group .The Xiao Yao San group increase faster than model group ,they have a statistical significance ( P<0 .05 or P<0 .01 ) .The model group showed a significant decrease in the Ghrelin level con-tents in both the blood and stomach bottom compared to the Xiao Y ao San and the normal groups ( P<0 .05 ) ,but the Xiao Yao San group had a higher level of Ghrelin in stomach bottom than model group ( P<0 .05) .Normal groups had similar Ghrelin level contents in stomach bottom;The model group had a lower level of Ghrelin in the blood than Xiao Y ao San group ( P<0 .01) .Conclusion Xiao Yao San can effectively treat rats with immobilization stress induced liver depression and spleen deficiency condition by raising the levels of Ghrelin in both the blood and stomach bottom .