Objective To investigate the efficacy of in vetro autologous HBsAg polypeptide-induced dendritic cells (DCs) in treating chronic hepatitis B(CHB). Methods On the basis of conventional hepatoprotective therapy, 32 CHB patients( group T) were treated with in subcutaneous infusion HBsAg polypeptide-induced DCs, which was reinfused intravenously. The efficacy was evaluated 3 months later and compared to that in 30 CHB cases (group C) treated with conventional therapy alone. Results The proportion of lymphocyte subsets,liver function tests,and negative rate of HBeAg/HBeAb were all improved more in group T than those in group C(P<0. 01). The difference in HBV-DNA conversion was not significant between two groups. The decrease of HBV-DNA and the levels of CD4+ CD8+ cell were more in group T than those in group C(P<0. 05). Conclusion Reinfusion of in vetro autologous HBsAg polypeptide-induced DCs has inhibitory effect on CHB through regulating immune tolerance.%目的 探讨体外诱导自体HBsAg多肽刺激的树突状细胞(DCs)治疗慢性乙型肝炎(CHB)的作用.方法 治疗组CHB患者32例,应用常规保肝治疗并皮下注射回输体外诱导自体HBsAg多肽刺激的DCs,治疗后3个月时评估疗效.同期CHB患者30例作为对照组.结果 治疗组T淋巴细胞亚群比例、肝功能指标、HBeAg阴转率和HBeAb阳转率均较对照组明显改善,HBVDNA阴转率无明显差异.治疗组HBV DNA下降量和CD4+、CD8+细胞水平明显高于对照组.结论 回输自体HBsAg多肽刺激的DCs可能通过调节免疫耐受,起到抑制乙型肝炎慢性化的效果.
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