玉米赤霉烯酮残留检测ELISA试剂盒的研制

摘要

A kit for testing zearalenone (ZEN) residues in feed was developed through the establishment of ELISA based on the obtaining of monoclonal antibody against ZEN.The linear detection of the kit was 5.0-405.0 μg/L (r =0.990 0),with the IC50 of 25.1 μg/L and the detection limit of 93.5 μg/kg.The ZEN kit had a good cross-reactivity with zearalenone,zearalanone,α-zearalenol,and zeranol.The recoveries of ZEN spiked in feed were 74.5％-109.9％ with the coefficient of variation less than 12.9％.The kit could be saved in 4 ℃ for 12 month.All the results indicated that the ZEN kit was suitable for the rapid detection of ZEN residues due to its sensitivity,specificity,and convienience.%为了建立饲料中玉米赤霉烯酮(ZEN)的ELISA试剂盒检测方法,本试验合成了ZEN半抗原,并进一步合成抗原(ZEN-BSA、ZEN-OVA),最终获得了抗ZEN单克隆抗体,在此基础上建立了ZEN残留检测的酶联免疫方法,并研制试剂盒.对试剂盒各项技术指标进行验证,结果显示:试剂盒的线性检测范围为5.0 ～ 405.0 μg/L,线性相关系数r=0.990 0,IC50浓度为25.1 μg/L,最低检测限为93.5 μg/kg,试剂盒对玉米赤霉烯酮、玉米赤霉酮、α-玉米赤霉烯醇、玉米赤霉醇均有较高交叉反应率,对饲料样品的回收率为74.5％ ～ 109.9％,样品检测变异系数小于12.9％,试剂盒可在4℃环境下保存12个月,具有较高的稳定性.表明该试剂盒具有快速、灵敏、特异、简便等特点,适合于ZEN残留的快速检测,具有较高的推广应用价值.