为了更好地理解水稻早熟性的遗传机制，对隐性早熟突变体ref进行了遗传分析和基因定位。突变体ref对光周期不敏感，与6个晚熟品种（系）杂交F1代均为晚熟。 ref×嘉禾218 F2分离群体抽穗期呈现连续双峰分布，早抽穗和晚抽穗之比符合1∶3的分离比，表明ref早熟性主要受1对隐性早熟基因控制。 F2群体中1005株早熟和晚熟单株组成定位群体，混合基因池分析发现5号染色体上SSR标记RM7302和RM3853与突变体ref早熟基因连锁。构建5号染色体的连锁图谱，进行表型和标记基因型的连锁分析，进一步确认突变体ref早熟基因定位在标记RM7302和RM3853之间18.32 cM的遗传区间内。%To uncover the genetic mechanism regulating early flowering in rice, a newly identified mutant ref was ge-netically analyzed and mapped for earliness genes. Ref was insensitive to photoperiodic response. The F1 progenies, derived from ref and six late flowering accessions, expressed late flowering. The heading date in ref × Jiahe218 F2 population dis-played continuous and bimodal frequency distributions, and the ratio early flowering plants to late flowering plants followed a 1∶3 segregation ratio, suggesting that earliness was controlled by a pair of recessive genes. A total of 1 005 early/late flowering plants derived from F2 generation was selected to construct the gene mapping population. Bulked segregation analy-sis identified SSR markers RM7302 and RM3853 on chromosome 5 associated with earliness genes. With further linkage a-nalysis, earliness gene was finally mapped to an interval of 18. 32-cM region between SSR markers RM7302 and RM3853 on the genetic map of chromosome 5.
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