乳胶过敏原Hev b 1二级结构及抗原表位的预测

摘要

Objective To predict the secondary structure and B/T cell epitopes of Hev b 1 protein for latex by application of bioinformatics software.Methods The amino acid sequence of Hev b 1 was obtained from the Uniprot database.The secondary structure of Hev b 1 was predicted by the GOR 4 online server.DNAStar Protean was applied to predict the B cell epitopes by analyzing the hydrophilicity,surface probability,flexible regions and antigenic index of Hev b 1,and the T cell epitopes were predicted through the NetMHC Ⅱ 2.2 server.Results The dominant B cell epitopes of Hev b 1 were on the regions of 1-14 and 40-46 and the dominant T cell epitopes were on 56-64,76-84 and 97-116.Conclusions We obtain the dominant B/T cell epitopes of latex allergen Hev b 1 by bioinformatic analysis,which lays a solid foundation for designing antibodies and modifying allergenicity of Hev b 1.%目的 利用生物信息学软件预测乳过敏原Hev b 1的二级结构及B细胞和T细胞的抗原表位.方法 在Uniprot数据库中获得Hev b 1蛋白序列,通过GOR 4在线软件预测其二级结构；采用DNAStar的Protean模块预测Hev b 1蛋白的亲水性、表面可及性、柔韧性及抗原指数,从而综合预测其B细胞表位；应用NetMHCⅡ2.2在线服务器预测Hev b 1蛋白的T细胞表位.结果 乳胶过敏原Hev b 1的B细胞优势抗原表位为1-14,40-46；T细胞优势抗原表位为56-64,76-84,97-116.结论 通过生物信息学分析获得乳胶过敏原Hev b 1的B细胞和T细胞优势抗原表位,可为乳胶过敏原Hev b 1的抗体设计、过敏原性改造等研究打下坚实的基础.