热应激和热惊厥幼龄大鼠海马及大脑皮层CCK蛋白表达研究

摘要

目的 探讨热应激(heat stress,Hs)和热惊厥(febrile convulsions,FC)对大鼠海马和大脑皮层胆囊收缩素蛋白(cholecystokinin,CCK)表达的影响.方法 采用热水浴诱导21日龄大鼠FC模型,应用免疫组织化学技术对HS和FC大鼠CCK在海马和大脑皮层的表达进行分析.结果 对照组中,大脑皮层偶见CCK零星表达.HS组CCK在大脑皮层有较弱表达,主要分布于杏仁周皮质、内嗅皮质和压部后皮质,海马未见表达.而FC组则于大脑皮层、海马见大量CCK强阳性细胞,呈弥漫性分布,无明显核团分布特征.结论 CCK在FC组中大脑皮层和海马各区明显表达,表明CCK参与海马等前脑结构对惊厥活动的中枢调控机制.%Objective To analyze the expression of cholecystokinin(CCK) in rat' s hippocampus and cerebral cortex following heat stress and febrile convulsions (FC). Methods Warm water was used to induce FC model. Immunohistochemistry method tested CCK protein. Results Very few or no CCK neurons were seen in the control rats. There was weaker expression of CCK in cerebral cortex of HS group,mainly located in PIR,ENT and RS subfield.No CCK positive ceiis were seen in hippocampus of HS rats. In contrast, abundant expression of CCK protein was found in hippocampus and cerebral cortex of FC group. They were well-distributed, without characteristic subnucleus-specific distribution. Conclusion Abundant induction of CCK in hippocampus and cerebral cortex of FC group suggested that CCK may play a role in the central control of FC.