Objective To evaluate the performance of chemiluminescence immunoassay( CLIA) and enzyme-linked immunosorbent assay( ELISA) for the detection of hepatitis C virus( HCV)antibodies. Methods Serum anti-HCV antibodies were detected in 2 460 hospitalized patients by CLIA and ELISA. F'urthermore,serum specimens with positive or discordant results were detected by Chiron RIBA HCV 3. 0 SIA to confirm the presence of anti-HCV. Results 57 samples with positive or discordant results were identified by CLIA/FLISA. 51 sample were positive,detected by CLIA,among which 49 samples were positive and 1 sample with discordant result were negative, confirmed by RIBA. In the 52 samples with positive results detected by by FLISA,47 samples were positive and 2 samples with discordant results were positive, confirmed by RIBA. The sensitivity of CLIA and FLISA were 100. 00 ％ and 95. 92％ , with the specificity of 99. 92％ and 99. 79％ , respectively. Conclusion CLIA could be more accurate and sensitive than ELISA in the initial HCV screening tests. Furthermore,to eliminate falsepositive reports, RIBA should be performed as additional confirmatory method.%目的 探讨化学发光法(CLIA)和酶联免疫吸附试验(ELISA)对丙型肝炎病毒(HCV)抗体的检测意义.方法 分别用CLIA法和ELISA法检测2 460例血清样本,收集CLIA/ELISA法检测阳性及可疑血清标本,以重组免疫印迹法(RIBA)最终确认.结果 两种方法共筛选57例CLIA/ELISA法检测阳性及可疑血清标本.CLIA法筛选51例阳性,RIBA确证49例,1例可疑标本经确证为阴性;ELISA法筛选52例阳性,RIBA确证47例阳性,有2例可疑标本确证为阳性.CLIA法和ELISA法敏感性分别为100.00%、95.92%;特异性分别为99.92%、99.79%.结论 CLIA法较ELISA法准确、灵敏,应用CLIA法可减少漏检现象.对ELISA法和CLIA法结果S/CO较低的标本均应慎重,必要时用RIBA法补充确证,以排除假阳性.
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