致外阴阴道假丝酵母菌病白假丝酵母菌对9种抗真菌药物最低抑菌浓度测定

摘要

目的 检测引起外阴阴道假丝酵母菌病(VVC)的白假丝酵母菌对9种抗真菌药物的耐药性,为临床治疗VVC提供参考依据.方法 常规培养分离真菌,并鉴定到种,采用最低抑菌浓度法对白假丝酵母菌进行体外药物敏感试验.结果 培养分离的41株菌中,白假丝酵母菌占87.8%,光滑假丝酵母菌占4.87%,季也蒙假丝酵母菌占2.43%,近平滑假丝酵母菌占2.43%,热带假丝酵母菌占2.43%;36株白假丝酵母菌耐药率由高到低依次是伊曲康唑、氟康唑、咪康唑、酮康唑、益康唑、5-氟胞嘧啶、克霉唑、两性霉素、制霉菌素,其中7种药物耐药率大于50%.结论 白假丝酵母菌仍是造成VVC的最常见致病菌,耐药现象较为普遍,应根据临床实验室的体外药敏试验结果,指导临床合理用药.%Objective To detect the 9 antifungal drugs' MIC of Candida albicans,isolated from vulvovaginal Candidiasis,and provide reference to VVC treatment. Methods The Candida were cultured and identified conventionally. The medical susceptibility test was performed by MIC methods to Candida albicans. Results Among 41 strains of Candida,the most common organisms were Candida albicans ( 87.8 ％ ), then Candida glabrata (4.87 ％ ), Candida guilliermondii (2.43 ％ ), Candida parapsilosis (2.43 ％ ), Candida tropicalis(2. 43％ ). Drug resistance rate from high to low was itraconazole, fluconazol, miconazole, ketoconazole, econazole,flucytosine,clotrimazole,amphotericin and mycostatin respectively,and the drug resistance rate was greater than fifty percent in 7 drugs of total. Conclusion Candida albicans is the most common cause of vulvovaginal candidiasis,and multi-drug resistance is gereral. It is important to use the antifungal drug according the result of drug sensitive test.