移居高原早期血浆同型半胱氨酸和多巴胺浓度的变化规律

摘要

目的 探讨健康青年男性移居高原早期血浆同型半胱氨酸(Hcy)和多巴胺(DA)浓度的变化规律.方法 在海拔1 400m某地选取10名拟进入海拔4 200 m高原的健康青年男性作为试验组,采用HPLC和LC/MS技术检测其进入高原后0、7、30 d血浆Hcy和DA浓度.同时选取职业、年龄相匹配的10名从平原移居海拔4 200 m 高原90 d的健康男性作为对照组,测定其上述指标.结果 试验组移居高原后血浆Hcy浓度先升高后降低,7 d时血浆Hcy浓度显著高于0 d的浓度,试验组移居高原30 d和对照组移居高原90 d时的血浆Hcy浓度均低于平原水平,差异均有统计学意义(P<0.05);试验组移居高原后血浆DA浓度也是先升高后降低,试验组移居高原30 d和对照组移居高原90 d时,血浆DA浓度显著低于试验组移居高原7 d时的浓度,差异均有统计学意义(P<0.05).结论 在高原习服过程中,血浆Hcy和DA浓度发生了变化以适应高原环境.%Objective To investigate the changes of homocysteine(Hcy)and dopamine(DA)in the plasma in healthy young men,who moved to the plateau. Methods Selected 10 men in a 1 400 m place,5 mL of venous blood(0 d)was collected,and the plasma Hey and DA were detected by high performanee liquid chromatography or mass spectrometry. Tracking these 10 people reached 4 200 m altitude,respectively,after reaching the plateau 7 d,30 d and then the Hcy and DA were measured. Also selected 10 work conditions,age matched men,who moved to 4 200 m for 90 d, these indexes was measured. Results Plasma level of Hcy in day 0[ (8. 17± 2.25 ) μmol/mL]was significantly lower than that in day 7[ ( 10. 84 ± 0.94 ) μmol/mL], P＜0.05, but significantly higher than that in day 30[(4. 58±2. 14)μmol/mL] and day 90[(1.29±0. 52)μmol/mL],P＜0. 05. Plasma level of Hcy in day 7 was significantly higher than that of day 30 and day 90(P＜0.05), the plasma in day 30 was greater than day 90(P＜0. 05) also. Plasma level of DA in day 7[(3. 233 ±0. 908) pg/μL]was significantly higher than day 30[(2. 495± 0. 205) pg/μL], P＜0.05 and day 90[(2. 39±0. 204)pg/μL],P＜0.05. Conclusion After moved to high land, Hcy and DA in the plasma can changed to adapt the hypoxis conditions.