上海市学龄前儿童末梢血血常规的参考值范围调查分析

摘要

目的：调查和了解上海地区学龄前儿童（2～6岁）血常规各项参数的正常参考值范围，为临床诊断提供参考。方法使用 Sysmex XS-800I 自动血液分析仪及原装配套试剂对7692例体检正常的学龄前儿童进行末梢血血常规分析，检测白细胞、红细胞、血小板、血红蛋白、红细胞压积、红细胞平均体积、红细胞平均血红蛋白水平和红细胞平均血红蛋白浓度；不同年龄组之间各项参数进行统计学分析，得出各项参数的参考区间。结果上海地区学龄前儿童血常规中，红细胞、血红蛋白、红细胞压积、红细胞平均血红蛋白水平、红细胞平均血红蛋白浓度、红细胞平均体积在性别上比较差异有统计学意义（P ＜0．05）；而白细胞和血小板计数性别上比较，差异无统计学意义（P ＞0．05）。该次调查得到的各参数参考值范围与仪器说明书给出的参考值范围相比存在较大差异。与相关报道的学龄前儿童末梢血血常规参考值范围相比部分参数存在差异，该研究白细胞计数参考值上限与几个地区的研究结果相比为最高，红细胞平均体积、红细胞平均血红蛋白水平和红细胞平均血红蛋白浓度参考值范围均高于其他地区研究结果。结论上海地区2～6岁学龄前儿童应该制定独立的血常规医学参考值范围，制定时也应该考虑到性别、仪器和试剂等因素的影响。%Objective To investigate and understand the reference value range of hematological parameters for peripheral blood routine in preschool children from Shanghai.Methods The Sysmex XS-800i automated hematology analyzer and the original rea-gents were used to measure the hematological parameters in peripheral blood samples collected from 7 692 healthy preschool chil-dren aged 2-6 years in Shanghai,including white blood cells(WBC),red blood cells(RBC),platelet(PLT)count,hemoglobin(Hb), hematocrit(HCT),mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH)and mean corpuscular hemoglobin con-centration(MCHC);the various parameters in different age groups were collected and statistically processed to establish the refer-ence intervals for each parameter.Results In the blood routine among preschool children in Shanghai,there were statistically signif-icant differences in all parameters except WBC and PLT count between sexes among preschool children in Shanghai(P <0.05).The reference intervals of hematological parameters obtained in this investigation were obviously differed from those offered by the man-ufacturer.However,compared with those from the related reports,the difference existed in partial parameters,the upper limit of WBC count was highest compared with the results from some areas,while the reference value ranges of MCV,MCH and MCHC were higher than those of other area study results.Conclusion The independent blood routine medical reference value range for preschool children in Shanghai should be established and the influence of the factors of gender,instrument and reagents also should be taken into consideration in establishing the reference value range.