Objective To survey and analyze the routine reference intervals of peripheral blood determination among healthy children. Methods A total of 2 408 healthy children from 2 to 6 years old were classified into several groups (2-,3-,4- and 5-) , and the white blood cell, red blood cell, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration of peripheral blood were determined. Reference intervals were obtained by statistical analysis of the parameters among the different age groups. Results Among the different age groups, the white blood cell was statistically significant (P < 0. 05). However, separate reference intervals were not estimated, unless the difference was at least 25% between the subclass means and combination means. The other parameters had no statistical significance ( P > 0. 05 ) . After the combination of the above parameters, the interval of white blood cell was 4. 8-11.6(7. 7) x 109/L, the interval of red blood cell was 3. 75-5. 18(4.47) x 1012/L, the interval of hemoglobin was 110-145 ( 126) g/L, the interval of hematocrit was 31. 24-1. 5 (36. 3)% , the interval of mean corpuscular volume was 76-88(81 )fl, the interval of mean corpuscular hemoglobin was 25-31(28)pg, the interval of mean corpuscular hemoglobin concentration was 329-368 (349) g/L, and the interval of platelet was 175-160(310) x 10 /L. The reference intervals of hemoglobin, red blood cell and hematocrit in Shanghai were similar to those in Changsha,and were lower than those in Kunming. The low and high limits of platelet in Shanghai were higher than those in Changsha and Kunming. Conclusions Different detection system should be established for the reference intervals in the different regions and different laboratories.%目的 调查分析本实验室健康儿童末梢血血常规参数的参考区间.方法 对体检合格的2 408名2～6岁儿童进行年龄分组(2-、3-、4-、5-),并做末梢血血常规分析,检测白细胞、红细胞、血红蛋白、红细胞压积、红细胞平均体积、红细胞平均血红蛋白含量和红细胞平均血红蛋白浓度等参数,不同年龄组之间各项参数做统计学分析,得出各项参数的参考区间.结果 不同年龄组之间均数比较,白细胞参数差异有统计学意义(P＜0.05),但各组均数与总体区间均数间相差＜25％,不建议分组,其他各项参数差异无统计学意义(P＞0.05),上述各项参数合并后白细胞为4.8～11.6(7.7) ×109/L,红细胞为3.75 ～5.18(4.47) ×1012/L、血红蛋白为110 ～145(126)g/L、红细胞压积为31.2 ～41.5(36.3)％、红细胞平均体积为76 ～88(81)fl、红细胞平均血红蛋白含量为25～31(28)pg和红细胞平均血红蛋白浓度为329 ～ 368( 349) g/L、血小板为175 ～ 460(310)×109/L.血红蛋白、红细胞、红细胞压积等参考区间与长沙儿童末梢血作比较基本一致,但低于昆明儿童末梢血,血小板参考区间的上限和下限均高于长沙、昆明.结论 不同地区、不同实验室应建立各自检测系统的参考区间.
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