HLA-B27抗原检测在强直性脊柱炎诊断中的意义

摘要

目的：探讨 HLA-B27抗原表达与强直性脊柱炎（AS）的相关性，及其对 AS 诊断和鉴别诊断的意义。方法采用流式细胞术检测人类白细胞抗原 B27（HLA-B27），纳入研究的包括354例疑似 AS 患者、35例确诊 AS 患者和30例确诊类风湿性关节炎（RA）患者。结果354例疑似 AS 患者的 HLA-B27总阳性率为22．0％，确诊 AS 患者 HLA-B27的阳性率为85．7％，均高于RA 组（P ＜0．05）。AS 患者中男性占77．1％，女性占22．9％，且发病年龄主要集中在青壮年时期；AS 患者 HLA-B27抗原检测用于AS 诊断的灵敏度为85．7％，特异度为100．0％。结论HLA-B27抗原的检测对 AS 患者的早期诊断和鉴别诊断具有重要意义。%Objective To investigate the correlation between the expression of human leukocyte antigen B27(HLA-B27)antigen and ankylosing spondylitis(AS),and its implications for the diagnosis and differential diagnosis of AS.Methods Flow cytometry was used to detect HLA-B27.354 suspected AS patients,35 patients final diagnosed with AS and 30 patients diagnosed with rheu-matoid arthritis(RA)were enrolled in the study.Results The positive rate of HLA-B27 in 354 patients with suspected AS was 22.0%,in patients diagnosed with AS was 85.7%,which was higher than the RA group(P <0.05).In AS patients,Males accoun-ted for 77.1%,females accounted for 22.9%,and the patients′age mainly concentrated in youth.The sensitivity and specificity of HLA-B27 test was 85.7% and 100.0% respectively in the diagnosis of AS.Conclusion HLA-B27 antigen detection is important for early diagnosis and differential diagnosis of AS patients.