荧光定量PCR染料法检测强直性脊柱炎患者HLA-B27基因及分型

摘要

目的 探讨荧光定量PCR染料法检测强直性脊柱炎(AS)患者人类白细胞抗原B27(HLA-B27)基因及亚型的临床应用价值.方法 收集2014年1月至2015年3月深圳市福田区风湿病专科医院已确诊的强直性脊柱炎患者血液标本43例和体检健康者血液标本56例,采用流式细胞法检测 HLA-B27基因,同时采用荧光定量 PCR 染料法检测 HLA-B27 基因及分型.结果 流式细胞法检测 43 例 AS 患者中 40 例阳性(93 .02%) ,荧光定量PCR染料法检测39例阳性(90 .70%) ,两者符合率为97 .50%. 39例 HLA-B27者中B2704亚型者有32例(82 .05%) ,B2705亚型者有7例(17 .95%) .结论 荧光定量PCR染料法检测 HLA-B27基因准确率高,与流式细胞法有高度一致性,同时还能检测HLA-B27基因亚型,具有很好的临床推广价值和应用前景.%Objective To investigate the clinical application value of fluorescence polymerase chain reaction (PCR) .in the human leucocyte antigen-B27(HLA-B27) gene and gene typing detection of ankylosing spondy-litis (AS) patients .Methods A total of 43 clinical blood samples of AS and 56 samples of healthy controls were collected in Shenzhen Futian hospital for rheumatic diseases from January 2014 to March 2015 .HLA-B27 gene was detected by flow cytometry .HLA-B27 gene and gene typing was also detected by the fluorescence PCR method .Results Among 43 samples ,40 samples were HLA-B27 positive(93 .02%) by flow cytometry while 39 samples were HLA-B27 positive (90 .70%) by fluorescence PCR .The total coincidence rate was 97 .50% .Among 39 positive samples ,32 samples were HLA-B2704 positive (82 .05%) and 7 samples were HLA-B2705 positive (17 .95%) .Conclusion The fluorescence PCR is an accurate method to detect HLA-B27 gene and presents high consistency with flow cytometry .It can also detect the HLA-B27 gene typing .It may have great clinical application value and prospects .