PCR-SSP法、FCM法及MLCT法检测强直性脊柱炎患者HLA-B27对比分析

摘要

目的：探讨序列特异性引物--聚合酶链反应（PCR-SSP）法、流式细胞仪（FCM）法及微量淋巴细胞毒（MLCT）法检测血清人类白细胞抗原-B27（HLA-B27）对强直性脊柱炎的诊断价值。方法：将58例确诊的强直性脊柱炎患者作为观察组，58例健康体检者作为对照组，分别采用PCR-SSP法、FCM法及MLCT法检测血清HLA-B27，分析3种方法诊断强直性脊柱炎的灵敏度、特异度、阳性预测值及阴性预测值。结果：PCR-SSP法、FCM法、MLCT法检测血清HLA-B27诊断强直性脊柱炎的灵敏度分别为96.55%、93.10%、82.76％，特异度分别为94.83%、91.38%、93.10%；PCR-SSP法、FCM法的灵敏度显著高于MLCT法，差异有统计学意义（P<0.05）；3种方法均有较高的特异度，差异无统计学意义（P>0.05）；PCR-SSP法具有较高灵敏度和特异度。阳性预测值分别为94.92%、91.53%、92.31%，阴性预测值分别为96.49%、92.98%、84.38%。3种方法均有较高的阳性预测值，差异无统计学意义（P>0.05）；PCR-SSP法、FCM法的阴性预测值显著高于MLCT法，差异有统计学意义（P<0.05）；PCR-SSP法具有较高的阳性预测值和阴性预测值。结论：3种检测方法对诊断强直性脊柱炎均有较高的特异度和阳性预测值，PCR-SSP法具有较高灵敏度、特异度、阳性预测值和阴性预测值。%Objective:To investigate the value of detecting HLA-B27 with PCR-SSP method,FCM method and MCLT method in the diagnosis of ankylosing spondylitis(AS).Methods:Selected 58 cases of AS patients as the observation group and 58 healthy subjects as the control group.HLA-B27 was detected respec-tively using the PCR-SSP method,FCM method and MLCT method,analyzing the diagnoses of the sensitivi-ty,speciifcity,positive predictive value and negative predictive value of AS with 3 methods.Results:By detecting HLA-B27 with PCR-SSP method,FCM method and MLCT method,the sensitivity of AS were 96.55%,93.10%and 82.76%,and speciifcity was 94.83%,91.38% and 93.10% respectively;the sensitivity of PCR-SSP method and FCM method was signiifcantly higher than that of the MLCT method,the difference being statistically signiifcant (P< 0.05);the 3 methods all had a higher speciifcity,there being no signiifcant difference (P> 0.05);PCR-SSP method had higher sensitivity and speciifcity.The positive predictive value were 94.92%,91.53%,92.31%,the neg-ative predictive value was 96.49%,92.98%,84.38% respectively.The positive predictive values of the 3 methods were high,and there was no signiifcant difference (P> 0.05);the negative predictive values of PCR-SSP method and FCM method were signiifcantly higher than that of the MLCT method,the difference being statistically sig-niifcant (P< 0.05);PCR-SSP method had higher positive predictive value and negative predictive value.Conclusion:The 3 kinds of detection method had a higher speciifcity and positive predictive value in the diagnosis of AS.The PCR-SSP method had higher sensitivi-ty,speciifcity,positive predictive value and negative predictive value.