铜绿假单胞菌痰培养阳性患者抗菌肽LL-37、CRP水平变化及临床意义

摘要

Objective To investigate the changes of antimicrobial peptide LL-37 and C-reactive protein (CRP ) in the patients with Pseudomonas aeruginosa positive sputum culture and their mutual relation . Methods Fifty cases of Pseudomonas aeruginosa positive sputum culture and 27 cases undergoing physical ex-amination in the Guangdong Provincial Hospital of Chinese Medicine from September 2016 to May 2017 were selected as the research subjects .The level of antimicrobial peptide LL-37 was detected by double antibody sandwich ELISA and the CRP level was detected by immunoturbidimetry .Results The levels of antimicrobial peptide LL-37 and CRP in the Pseudomonas aeruginosa positive sputum culture group were significantly high-er than those in the healthy control group ,the difference was statistically significant (P< 0 .05) ,and they showed the positive correlation (r=0 .411 ,P<0 .05) .Conclusion In positive sputum culture of Pseudomonas aeruginosa ,antimicrobial peptide LL-37 and CRP levels are increased ,which has a certain clinical application value for the early diagnosis of infection .%目的 探讨铜绿假单胞菌痰培养阳性患者的抗菌肽LL-37和C反应蛋白(CRP)水平变化情况及相互关系.方法 选取广东省中医院2016年9月至2017年5月收治的铜绿假单胞菌痰培养阳性患者50例、健康体检27例作为研究对象,采用双抗体夹心酶联免疫吸附试验法检测抗菌肽L L-37水平,免疫比浊法检测CRP水平.结果 铜绿假单胞菌痰培养阳性患者组的抗菌肽LL-37和CRP水平均显著高于健康对照组,差异有统计学意义(P<0.05),两者呈正相关(r=0.411,P<0.05).结论 铜绿假单胞菌痰培养阳性时,抗菌肽LL-37和CRP水平升高,对评估患者感染的早期诊断有一定临床应用价值.