· AIM: To quantitatively detect gene expression level of transforming growth factor-β type 1 receptor (Tβ R 1) and transforming growth factor-β type 2 receptor (Tβ R 2) in different stage of diabetic rats' retina. To observe and analyze the effect of transforming growth factor-βreceptors on the retina of rat diabetic animal model.· METHODS: 28 healthy adult Sprague-Dawley rats were chosen and randomly divided into two groups of normal control (CON) and diabetes mellitus (DM). Diabetes was induced by streptozotocin (STZ) intraperitoneal injection.Gene expression was detected quantitatively with real-time fluorescence quantitative reverse transcription polymerase chain reaction (QRT-PCR).· RESULTS: The mRNA level of Tβ R 1 and Tβ R 2 was 0.000493± 0.000133 and 0.000166± 0.000057 at 4wk. The mRNA level of Tβ R 1 and Tβ R 2 was 0.000608± 0.000232 and 0.000113± 0.000049 at 12wk. Tβ R 1 expression was gradually elevated during the progression of diabetic retinopathy, Tβ R 2 expression was up-regulated at 4wk,but down-regulated at 12wk.Tβ R 2) may play important role in the pathogenesis of diabetic retinopathy.%目的:定量检测转化生长因子β受体-1(Transforming Growth Factor-β receptor type 1,Tβ R 1)和转化生长因子β受体-2(Transforming Growth Factor-β receptor type 2,T β R 2)编码基因在糖尿病大鼠视网膜中不同时相点的动态表达水平,以探讨转化生长因子β及转化生长因子β受体在糖尿病视网膜病变(DR)中的作用.方法:选择健康成年SD大鼠28只,随机分为正常对照组(CON)和糖尿病组(DM).利用链尿佐菌素(STZ)诱导大鼠糖尿病视网膜病变模型,制备RNA并逆转录,实时荧光定量PCR技术分析TβR 1和TβR 2在不同时相点相对于内参照基因18s的相对mRNA含量.结果:TβR 1在4,12wk时相对于18s的mRNA含量分别为0.000493±0.000133和0.000608±0.000232.TβR 2在4,12wk时相对于18s的mRNA含量分别为0.000166±0.000057和0.000113±0.000049.TβR 1基因表达水平随着病变进展呈上升趋势,TβR 2在4wk时表达量较正常增加,到12wk时表达量又减少,逐渐恢复到正常水平.结论:糖尿病大鼠视网膜中TβR 1和TβR 2基因表达水平变化可能影响TGF-β信号的转导过程,TGF-β及其受体介导的信号转导通路在糖尿病视网膜病变中具有重要作用.
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