微生物资源库中产纤维素酶菌株的筛选及其酶学性质研究

摘要

A bstract 112 strains producing cellulase w ere screened from 625 bacterial and actinom ycetes iso lates conserved in culture and inform ation cen ter of industrialm icroorganism of China universities (C IC IM -CU ) by iodine cobrations m ethod . These strains w ere identified based on 16S rDNA sequence analysis. T he results show ed that three new ly isolated producers of cellulase, including Com am onas testosterone, Paeniacillus tundrae and S trep torn yoes tanashiensis were screened . For the first tim e, cellulase produced by S ttep torn yoes tanashiensis w as reported in 1his study . The op tin al conditions for 1he enzym atic reactbn w ere about50 ℃ and pH 6 .0 . A tpH 5 .0~7 .0 , the enzym e show ed high activity and good stability . This new theim otolerable and pH stable cellulase m ay m ake a significant contribution to the cellulose related industry .%利用平板碘液染色法对中国高校工业微生物资源与信息中心(CICIM-CU)资源库中保藏的625株细菌和放线菌进行筛选,从中筛出112株具有纤维素酶产生能力的菌株,建立了一个产纤维素酶微生物资源库.对筛选得到的纤维素酶产生菌株进行16S rDNA鉴定后发现,其中的Streptomyces tanashiensis、Paenibacillus tundrae和Comamonas testosteroni等微生物产生的纤维素酶国内外未见文献报道.对Streptomyces tanashiensis CICIM B4515菌株产生的纤维素酶的酶学性质进行了初步研究,发现其最适反应温度为50 ℃,在60 ℃以下活性稳定;最适反应pH为6.0,在pH 5.0～7.0范围内酶活相对稳定.较好的温度耐受性和较宽的pH作用范围使得该菌株产生的纤维素酶具有一定的工业应用前景.