METHOD FOR RAPIDLY SCREENING MICROBIAL HOSTS TO IDENTIFY CERTAIN STRAINS WITH IMPROVED YIELD AND/OR QUALITY IN THE EXPRESSION OF HETEROLOGOUS PROTEINS

摘要

The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous polypeptides in improved yield and / or quality. The array includes one or more host cell populations genetically modified to either increase expression of one or more target genes involved in protein production, decrease expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in the surrounding cytoplasmic region or secrete the heterologous protein through the outer cell wall. The array of strains is useful for screening for enhanced expression of any protein of interest, including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines,;Heterologous protein expression, optimal host identification, screening method, Pseudomonas fluorescens