华根霉产纤溶酶液体发酵条件的研究

摘要

The optimization for liquid-state fermentation of Rhizopus chinensis TCCC 41014 producing fibrinolytic enzyme was carried out . The efects of carbon source , nitrogen source , initial pH , liquid volume and temperature on the fibrinolytic enzyme activity were investigated . The results showed that the appropriate medium composed were as follows : dextrin 80 g/L , peptone 60 g/L , bean meals 60 g/L and initial pH of 4 .5 . The optimized fermentation time ,temperature and inoculum volume were 60 h, 29℃ and 5×10" spores per mL , respectively . Under the optimized conditions the average fibrinolytic enzyme activity reached 64 .64 U/mL .%研究了华根霉TCCC 41014产纤溶酶液态发酵的工艺条件,采用单因素实验对液态发酵培养基的碳源、氮源、初始pH值、温度和装液量进行了优化.结果表明,实验范围内华根霉TCCC41014液态发酵产纤溶酶的适宜培养基组成(g桙L):糊精80,蛋白胨60,豆粕60,初始pH为4.5.适宜培养条件:培养温度为29℃,装液量为50/500mL 三角瓶,接种量5×105个孢子/mL,培养时间为60h.优化条件下的纤溶酶产量达64.64U/mL.