湖南省葫芦科作物小西葫芦黄花叶病毒的检测

摘要

根据已报道的小西葫芦黄花叶病毒（Zucchini yellow mosaic virus，ZYMV）保守序列设计了一对特异性引物，在58℃下退火30 s，建立了一种可靠、有效的ZYMV普通RT-PCR检测方法。通过该方法成功克隆出一条长度为621 bp的HC-Pro基因片段，并与杭州分离物的ZYMV辅助成分/蛋白酶（HC-Pro）基因核心区域（登录编号为AJ515911.1）的相似度达到99%。采用该方法对湖南省7个地区采集的疑似感病样品进行检测，结果显示：ZYMV平均阳性检出率达54.0%，说明ZYMV是侵染湖南省葫芦科作物的主要病毒之一。%According to the reported conserved sequence of zucchini yellow mosaic virus (ZYMV), a pair of speciifc primer was designed and then annealed at 58℃ for 30 s, thereby successfully establishing an effective and reliable RT-PCR detection method for ZYMV. Through the method, a 621 bp ofHC-Pro gene segment was cloned successfully, and the similarity between it and the core gene region of ZYMV HC-Pro isolated from Hangzhou samples (the login ID is AJ515911.1) reached 99%. Using the method to detect suspected infected samples collected from seven areas of Hunan province, the results showed that the average positive detection rate of ZYMV was as high as 54.5%. Therefore, the ZYMV is one of the main viruses which infested in the cucurbitaceous crops in Hunan province.