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Specific Primers for Detection of Zucchini yellow mosaic virus
Specific Primers for Detection of Zucchini yellow mosaic virus
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机译:用于检测西葫芦黄色花叶病毒的特定引物
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摘要
The invention jyukini sulfide mosaic virus species and relates to nucleic acid fragments which react specifically with, and more particularly, nucleotide sequences, its complementary nucleotide sequence or those nucleotide sequences shown in SEQ ID NO: 2,4,5,7,8 discloses in any one of the nucleic acid fragment or a primer consisting of a specific combination of the nucleic acid fragments of the modified sequence is a variation based embodiment.; According to the present invention is more than 1000 times higher than the detection limit diagnostic method using the anti-serum, is detected for all the strains of known jyukini hanghwa mosaic virus is possible so far. In addition, no nonspecific reaction with other related viruses can be much more accurately diagnose infection of jyukini sulfide mosaic virus.
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机译:本发明的久屈尼硫化物花叶病毒种,涉及与核苷酸序列,其互补核苷酸序列或SEQ ID NO:2、4、5、7、8中所示的那些核苷酸序列特异性反应的核酸片段。所述核酸片段或由所述修饰序列的核酸片段的特定组合组成的引物中的任一个是基于变异的实施方案;根据本发明,比使用抗血清的检出限诊断方法高出1000倍以上,到目前为止,对于已知的久留尼·杭华花叶病毒的所有菌株的检出都是可能的。另外,没有与其他相关病毒的非特异性反应可以更准确地诊断出久留尼硫化物花叶病毒的感染。
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