直接用纯种菌体在适当条件下培养得到菌体,消除了生物菌体混入杂菌的影响.通过正交试验得出海藻酸钠固定化鞘氨醇单细胞小球对COD降解的最佳条件;讨论了时间、温度、pH值、降解剂用量、摇床摇速几个单因素对海藻酸钠固定化鞘氨醇单胞菌小球降解COD的影响:通过比较可以得出在降解COD的过程中,微生物只有借助某一载体才能表现出其最佳降解活性,微生物包埋法具有操作简单,效果明显,污染少,成本低等优点.%Using pure bacteria to culture new thalli could eliminate the influence of other bacteria. The optimum degradation condition of sodium alginate immobilized Sphingom onas pellet to COD degradation was explored through the orthogonal experiment, and the influence of several single factors to the sodium alginate immobilized Sphingom onas pellet to COD degradation, including time, temperature, pH value, the degradation reagent dosage and the speed of the shaker were discussed. It was found that the microorganisms showed high degradation activity only by means of a certain vector during the process of COD degradation. Dynamics research indicated that among the several methods of the COD degradation, micro-organisms embedding had many advantages such as simple operation, obvious effect, less pollution, low cost, etc.
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