选用21日龄昆明系清洁级雄性小鼠40只.随机分为5个处理组,即正常对照组和感染对照组(基础日粮+生理盐水,Ⅰ组和Ⅱ组),伴大豆球蛋白组(基础日粮+伴大豆球蛋白,Ⅲ组),伴大豆球蛋白胃蛋白酶水解肽组(基础日粮+伴大豆球蛋白胃蛋白酶水解肽.Ⅳ组)和水解肽分离有效抑菌组分组(基础日粮+水解肽分离有效抑菌组分,Ⅴ组).预饲3 d后.除Ⅰ组和Ⅱ组外,其他各处理组灌喂液体总含氮量为10m异/mL,每只小鼠灌喂量均为0.5mL.连续灌胃10 d后,于第11天将10<'8>CFU/mL的沙门氏菌分别灌喂除正常对照组的其他各组(剂量均为0.5mL/只),24 h后采用摘除眼球法收集血液后,宰杀各组小鼠,取脾脏、肠黏膜,分析血清和脾脏中TNF-α、IL-6和肠黏膜组织中slgA的变化.结果表明在小鼠发生沙门氏菌感染时,伴大豆球蛋白水解肽以及水解肽分离有效抑菌组分能显著降低小鼠血清TNF-α水平(P<0.05)和脾脏IL-6水平(P<0.01),提高脾脏TNF-α和肠道黏膜sIgA水平(P<0.01).说明伴大豆球蛋白胃蛋白酶水解肽能提高动物机体免疫机能,抵御外源性沙门氏菌的侵袭感染,预防胃肠道疾病的发生.%40 male KM mice at age of 21 days were randomly divided into 5 groups. Two control groups ( Ⅰ , Ⅱ ), which were normal mice and Salmonella infected mice respectively, were fed with basic diet and saline. The other three groups were conglycinin group( Ⅲ ), conglycinin peptides group(Ⅳ ) and effective bacteriostats group (Ⅴ). The total nitrogen content fed to each group was 10mg/mL except group Ⅰ and Ⅱ. Each mouse was fed with 0.5 mL per day and after 10 days the mice in group Ⅱ, Ⅲ, Ⅳ, Ⅴ group was fed with 0.5mL Salmonella typhimurium (108CFU/mL). After 24 hours the blood,spleen and the intestinal mucosa were collected to analyze the variation of TNF-α and IL-6 in serum and spleen, and sIgA in intestinal mucosa. The results showed that the conglycinin hydrolytic peptides and its effective bacteriostats could significantly reduce the level of TNF-α in serum (P＜0.05) and IL-6 in spleen (P＜0.01) of the infected mice. They could also increase the level of TNF-α in spleen and sIgA in intestinal mucosa(P＜0.01 ). The results indicated that the conglycinin hydrolytic peptides could enhance the immunity function of animal, resist the invasion of Salmonella typhimurium, and prevent gastrointestinal tract diseases.
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