南海红树林内生真菌GX-3代谢产物研究

摘要

The secondary metabolite compounds of mangrove endophytic fungus GX-3 from the South China Sea were isolated by column chromatography and identified on the basis of physic-chemical constants and spectral analysis.Six metabolites were obtained and elucidated as cyclo-(Phe-Gly) (1),cyclo-(Leu-Gly)(2),Fusaric acid(3),3,6-di-sec-butyl-1,4-dihydroxypiperazine-2,5-dione (4),cyclo-(pro-leu)(5),cyclo-(Gly-Leu)(6).In the preliminary bioassay,1 showed strong inhibitory activity against KB,KBv200 with LD50 at 6.5 and 11.2 μmol/L,respectively.The six metabolites were obtained from endophytic fungus GX-3 for the first time.%采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等分离纯化南海红树林内生真菌GX-3的代谢产物,并通过理化常数测定和光谱分析鉴定其化学结构.结果表明,从南海红树林内生真菌GX-3的菌体中分离得到6个代谢产物.经波谱解析,分别为:环(苯丙-甘)二肽(1)、环(丙-亮)二肽(2)、Fusaric acid (3)、3,6-di-sec-butyl-1,4-dihydroxypiperazine-2,5-dione(4)、环(脯-亮)二肽(5)、环(甘-亮)二肽(6).初步药理活性显示化合物1对口腔癌细胞KB、KBv200的抗肿瘤活性的LD50值分别为6.5和11.2μmol/L.通过鉴定,所有化合物均系首次从南海红树林内生真菌GX-3中分离得到.