利用RNA干扰技术研究长牡蛎TLR2-2基因对MyD88-2基因表达的影响

摘要

为研究长牡蛎(Crassostrea gigas)免疫基因TLR2-2对MyD88-2基因表达的调控作用，将体外合成的dsRNA注射进入成体长牡蛎体内，72 h后检测TLR2-2基因和MyD88-2基因的表达量。结果表明，成功地对TLR2-2基因和MyD88-2基因进行了干扰，而在TLR2-2基因被干扰后，MyD88-2基因的表达量显著下降，但在MyD88基因被干扰的长牡蛎中TLR2-2基因表达量没有明显变化。%Double strands RNA synthesized in vitro transcription was injected to adult Pacific oysters (Crassostrea gigas), to study the interaction between TLR2-2 and its potential downstream MyD88 gene. The expression of TLR2-2 and MyD88-2 analyzed by qPCR reduced 72 h after injection. Moreover, in oysters where expression of TLR2-2 was inhibited by dsRNA, MyD88-2 was also found down-regulated, while the expression level of TLR2-2 in oysters where MyD88-2 was suppressed was not significant different compared with that of control group.