硼酸溶液细菌内毒素检查干扰实验

摘要

目的 建立硼酸溶液细菌内毒素检查方法.方法 按<中华人民共和国药典>2010年版附录细菌内毒素检查法,分别进行了鲎试剂灵敏度复核实验、细菌内毒素干扰实验预实验及正式实验.结果 硼酸溶液稀释4倍后,用灵敏度为0.125 EU·mL-1的鲎试剂进行检查,可消除抑制干扰.结论该方法简便可靠,可用于硼酸溶液细菌内毒素的检查.%Objective To establish a method for checking bacterial endotoxin in solution of boric acid. Methods The method was based on the bacterial endotoxin test described in the appendix of Chinese Pharmacopeia 2010, including the sensitivity of tachypleus amebocyte lysate ( TAL ), preliminary interference test and official interference test of bacterial endotoxin. Results The interference was eliminated by using 0. 125 EU ■ mL" TAL in boric acid solution being diluted by 4 times. Conclusion The method is simple and reliable, which can be used for checking bacterial endotoxin in boric acid solution.