Objective To establish gas chromatography ( GC) method for separating and determining both borneol and menthol in tanxiang tongmai capsule. Methods Naphthalene served as an internal standard, acetic ether as a solvent, 10% PEG-20M as separation, nitrogen as the carrier gas. Column temperature was set at 130℃ , and injector and detector temperature was 180℃. Sample size was 1 μL by using flame ionization detector. Results The linear ranges of borneol and menthol were 0.26-2.55 mg ? mL-1(r = 0. 999 9) and 0. 26 - 2. 62 mg ? mL-1(r = 0. 999 8) , respectively. Recovery rates of borneol and menthol were 97. 5 % (RSD = 1.77 % ) and 96. 81 % (RSD = 1.28 % ) , respectively. Conclusion This method is convenient, rapid, accurate and reproducible, and can be used for quality control of tanxiang tongmai capsule.%目的 建立气相色谱法同时测定檀香通脉胶囊中冰片、薄荷脑两组分的含量.方法 以萘为内标物质,乙酸乙酯作为溶剂,岛津CBP-M25-025(25 m×0.22 mm,0.25 μm)为色谱柱;载气氮气,柱温130 ℃,进样口和检测器温度均为180 ℃,氢火焰离子化检测器,进样量1 μL.结果 测定各组分达到良好分离,冰片的线性范围为0.26～2.55 mg·mL-1(r＝0.999 9),薄荷脑的线性范围为0.26～2.62 mg·mL-1(r＝0.999 8);冰片的平均回收率为97.5%(RSD＝1.77%),薄荷脑的回收率为96.8%(RSD＝1.28%).结论 该方法灵敏、快速、简便、可用于檀香通脉胶囊的质量控制.
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