百尾参药材高效液相色谱指纹图谱分析

摘要

目的 建立百尾参药材的高效液相色谱指纹图谱. 方法 采用高效液相色谱法,以Agilent Zorbax SB-C18 (250 mm×4.6 mm,5 μm)为色谱柱,以甲醇-0.05%磷酸为流动相,梯度洗脱,流速为1 mL·min-1,检测波长256 nm,柱温为30 ℃. 结果 建立了15批不同产地的百尾参药材指纹图谱,共标定13个分离度较好的共有峰,利用对照品指认4个共有峰;用聚类分析和主成分分析对指纹图谱进行化学模式识别研究. 结论 该方法简便、准确、重复性好,可作为百尾参药材质量控制的手段.%Objective To establish the HPLC fingerprint of Disporum cantoniense. Methods HPLC analysis was performed on Agilent Zorbax SB-C18 chromatographic column ( 250 mm × 4. 6 mm, 5 μm) with the mobile phase consisting of methanol-0.05% phosphoric acid in gradient mode.The flow rate was 1.0 mL·min-1, the detection wavelength was 256 nm and the column temperature was 30 ℃. Results The HPLC fingerprint of 15 batches of Disporum cantoniense was established. Thirteen common peaks in the fingerprint were demarcated, four of which were identified by reference substances. Chemical pattern recognition of fingerprint was performed by hierarchical cluster analysis and principal component analysis. Conclusion The method is simple, accurate and has a good repeatability, and can be used for quality control of Disporum cantoniense.