高效液相色谱法对不同种人参属药用植物的指纹图谱分析

摘要

目的 建立高效液相指纹图谱方法用于区分人参属不同商品药材包括三七,圆参(种植国产人参和种植高丽参),野山参,红参以及三种不同化学分型的西洋参,保证人参属药材的安全,正确使用.方法 采用Zorbax Extend C18(250 mm×4.6 mm,5μm)色谱柱,乙腈和10 mmol·L-1 KH2PO4水溶液为洗脱溶剂,建立人参属药材的液相指纹图谱方法.结果 根据人参属不同商品药材液相指纹图谱的整体和一些特殊成分的差异, 可以区分人参属不同商品药材.结论 高效液相色谱指纹图谱方法简单,有效,可重复,可以用于人参属不同商品药材的常规鉴别.%Aim To establish a method for differentiaing commercial samples of Panax species including notoginseng,cultivated ginseng(Chinese ginseng and Korean ginseng),wild ginseng,red ginseng,three types of American ginsengs,and one American ginseng preparation with their HPLC fingerprints for assnrning the quality of different commercial samples of Panax species.Methods HPLC-UV method was used to establish their fingerprints,Zorbax Extend C18(250 mm×4.6 mm,5μm)was used as the analytical column,and acetonitrile/KH2PO4 aqueous solution Was used as the mobile phase with gradient elution.Resuits The fingerprints of different commercial samples of Panax species varied in their holistic chromatograms and some specific constituents.Conclusion This method is reliable, reproducible and simple.It could be applied in the routine authentication of different commercial samples of Panax species