高效液相色谱法同时测定益气和胃胶囊中4种成分的含量

摘要

目的 建立益气和胃胶囊中4种成分的含量测定方法.方法 采用反相高效液相色谱(RP-HPLC)法,ThemoC18色谱柱(150 mm×4.6 mm,0.5μm),流动相为乙腈-水(用磷酸溶液调节pH值至3)(20:80);流速为1.0 mL·min-1,检测波长为283 nm.结果 黄芩苷在(0.5735~5.7360)×10-2 mg·mL-1、柚皮苷在(0.1940~1.9395)×10-2 mg·mL-1、橙皮苷在(0.2051~2.0510)×10-3 mg·mL-1、新橙皮苷在(0.2020~2.0200)×10-2 mg·mL-1范围内各自峰面积积分值均呈良好的线性关系;RSD分别为0.28%,0.54%,0.54%,0.31%(n=5).黄芩苷、柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为103.94%,98.06%,103.09%,95.67%.结论 该方法简便、灵敏、准确,可用于益气和胃胶囊的制剂质量控制.%Objective To establish a method for the quantitative determinations of the ingredients in yiqi hewei capsules. Methods RP-HPLC method was used. The separation was performed on a Thermo C18 column with mobile phase consisting of acetonitrile-water (20: 80) (pH was adjusted to 3 with glacial acetic acid) at the flow rate of 1. 0 mL·min-1. The detection wavelength was 283 nm. Results The linear ranges were ( 0. 5735 -5. 7360) × 10 -2 mg·mL-1 for baicalin, (0. 1940-1. 9395) × 10 -2 mg·mL-1 for hesperidin, (0. 2051 -2. 0510) × 10 -3 mg·mL-1 for naringin and (0. 2020 -2.0200) ×10 -2 mg·mL-1 for neohesperidin;RSD =0.28%,0.54%,0.54%,0.31%(n =5),respectively. The average recoveries were 103. 94%, 98. 06%, 103. 09%, 95. 67%, respectively. Conclusion The established method is simple, sensitive and accurate,which can be used for the quality control of yiqi hewei capsules.