目的 建立高效液相色谱(HPLC)法同时测定复方苯甲酸涂剂中苯甲酸和水杨酸含量.方法 采用Alltima C18色谱柱(150 mm×4.6 mm,5μm),以甲醇-0.1% 磷酸(50:50)为流动相,流速1.0 mL?min-1,检测波长236 nm,柱温35℃.结果 苯甲酸和水杨酸的线性关系良好,线性相关系数(r)均为1.0000;精密度和稳定性良好,RSD均<0.3%;回收率良好,苯甲酸和水杨酸的平均回收率分别为100.15%和99.85%(n=9).结论 该方法准确、简单、快速,可用于同时测定复方苯甲酸涂剂中苯甲酸和水杨酸含量.%Objective To develope a high performance liquid chromatography ( HPLC) method for simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid embrocation. Methods An Alltima C18 column (150 mm×4.6 mm,5 μm) was used for the separation, with methanol-0.1% phosphoric acid (50:50) as the mobile phase at the flow rate of 1.0 mL?min-1 .The detection wavelength was set at 236 nm.The column temperature was 35 ℃ . Results The good linearity was obtained with the correlation coefficient (r) of 1.0000 for benzoic acid and salicylic acid;the precisions and stability were satisfactory, and the relative standard deviations (RSDs) were less than 0.3%.The average recoveries (n= 9) were 100.15% and 99.85% for benzoic acid and salicylic acid, respectively. Conclusion The established method is accurate, simple and rapid, and can be utilized for the simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid embrocation.
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