目的 优选大孔树脂分离纯化马黛茶中多酚类物质组分最佳工艺条件.方法 采用高效液相色谱法建立马黛茶指纹图谱,以绿原酸和高效液相色谱指纹图谱共有峰的总有效成分洗脱率为指标,筛选最佳型号树脂、上样条件和洗脱条件.结果 以D101型大孔树脂为分离载体,样品液浓度为0.10 g?mL-1,树脂吸附量为生药1.5 g?g-1,先用4倍量水洗脱除杂质,再用4倍量70％乙醇梯度洗脱,纯化后提取物对绿原酸的洗脱率达82.83％,对总有效成分的洗脱率72.48％,高效液相色谱指纹图谱相似度大于0.98,成分均衡回收.结论 D101型大孔树脂纯化马黛茶中多酚类物质组的工艺简便可靠,可用于规模化生产.%Objective To study the optimum technological conditions of macroporous resin separation and purification of polyphenol composition. Methods Fingerprint of Ilex paraguariensis was established by HPLC method. The best resin, loading conditions and elution requirements were screened with chlorogenic acid and elution rate of total active constituents served as the index. Results The optimum technological conditions were as follows:the separation carrier was D101 macroporous resin,the concentration of the sample liquid was 0.10 g?mL-1 ,the resin adsorption quantity of crude drugs was 1.5 g?g-1 ,the impurity was removed by four times of amount of water at first,and then eluted with four times the amount of 70％ ethanol in a gradient manner.After purification,the elution rate of chlorogenic acid was up to 82.83％,and the elution rate of total effective component was about 72. 48％. The resemblance of HPLC fingerprint was over 0. 98, and the components were balancedly recoveried. Conclusion The purification process of total polyphenols in Ilex paraguariensis by D101 macroporous resin was simple,reliable,and can be used for large-scale production.
展开▼
