LGR5在宫颈癌发生中的作用及机制初探

摘要

Objective:To investigate the role and mechanism of LGR5 in cervical carcinogenesis. Meth-ods:The expression of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and squa-mous cervical cancer were determined by immunohistochemistry and western blot, and analyze the relationship between LGR5 and clinical pathological features of cervical cancer and β-catenin expression. The effect of LGR5 on cervical carcinogenesis was examined using tumorsphere formation. Results: The positive rate of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and cervical cancer were 17％ ( 5/30), 65％ (11/17), 84％ (54/64) and 13％(4/30),53％(9/17),92％(59/64),respectively. The differ-ence was statistically significant ( P<0.01) . The expression of LGR5 was associated with pathological grade of cervical cancer, but not with patient's age, clinical stage and lymph node metastasis. β-catenin expression in cervical cancer was positively related to LGR5 expression(r=0.69, P<0.01). Moreover, the rate of tumor-sphere formation of LGR5 overexpression SiHa cell was significantly higher than the GFP control group ( P<0. 01) . Conclusion:LGR5 and promoted cervical carcinogenesis and its mechanismmay be associated with high expressio0n of β--catenin%目的:研究富含亮氨酸重复序列的G蛋白偶联受体5(LGR5)在宫颈癌发生中的作用及机制.方法:分别采用免疫组化、Western blot检测LGR5、β-链蛋白(β-catenin)在正常宫颈、宫颈上皮内瘤变及宫颈癌组织中的表达,分析LGR5与宫颈癌临床病理特征、β-catenin的关系;通过肿瘤球形成试验观察LGR5对宫颈癌发生的影响.结果:LGR5、β-catenin在正常宫颈、宫颈上皮内瘤变及宫颈癌中的阳性率分别为17％(5/30),65％(11/17),84％(54/64)以及13％(4/30),53％(9/17),92％(59/64),差异均具有统计意义(P<0.01).LGR5表达与宫颈癌病理分级相关,而与患者年龄、临床分期及淋巴转移无相关性.宫颈癌组织中β-catenin与LGR5的表达呈显著正相关(r=0.69,P<0.01).LGR5过表达的人宫颈鳞癌细胞(SiHa细胞)肿瘤球形成率高于绿色荧光蛋白(GFP)对照组(P<0.01),差异具有统计学意义.结论:LGR5促进宫颈癌的发生,其机制可能与β-catenin高表达相关.