三氧化二砷对表皮癌细胞株A-431增殖及Survivin表达的影响

摘要

目的 探讨三氧化二砷(As2O3)对表皮癌细胞株A-431增殖和细胞周期的影响以及对Survivin基因表达的影响,为As2O3治疗表皮癌提供实验室依据.方法 培养表皮癌细胞A-431,加入不同浓度的As2O3,MTr法观察As2O3对A431细胞增殖的影响,应用流式细胞仪检测As2O3对A-431细胞细胞周期、凋亡和Survivin基因表达的变化.应用Western Blot免疫印迹技术观察As2O3对A-431细胞Survivin基因表达的影响.结果 从浓度10μmol/L到40μmol/L,As2O3可以显著地抑制A-431细胞的增殖,呈时间依赖性和浓度依赖性；As2O3影响A431细胞周期的分布,作用后G0/G1期期细胞群增多,凋亡数目增多(P＜0.05).与对照组比较,As2O3能抑制A-431细胞Survivin基因的表达,该蛋白量下降(P＜0.05).结论 As2O3对体外培养的表皮癌细胞株有显著的增殖抑制作用,有诱导表皮癌凋亡的作用；并可能与抑制凋亡抑制基因Survivin的表达有关.%Objective To explore the effects of arsenic trioxide (As2O3) on the cell proliferation and expression of Survivin in human epidermal carcinoma cell line-A 431 in vitro. Methods The human epidermal carcinoma cells (cell line A-431) were cultured in vitro .then the cells were treated with different concentrations of arsenic trioxide,then the effects of arsenic trioxide on the cell proliferation of A 431 were detected by MTT.the effects of arsenic trioxide on the cell cycle, apoptosis and expression of Survivin were measured by flow cytometry and the effects of arsenic trioxide on expression of Survivin were also tested by Western Blot. Results The As2O3 could obviously inhibit the proliferation of A 431 cells with concentrations from 10μmol/L to 40μmol/L,which was time-dependent and concentration-dependent. The As2 03 could affect the cell cycle of A 431 cells, with the increase of cell counts at G0/G1 phase and the increase of apoptotic cell counts ( P < 0. 05 ). As compared with control group,As2O3 could significantly inhibit the expression of Survivin of A 431 cells. Conclusion The As2O3 can obviously inhibit the proliferation of the human epidermal carcinoma cells cultured in vitro, and can induce the cells' apoptosis, which may be related to its inhibition effect on the expression of Survivin.