The effects and mechanism of down-regulation of survivin expression by RNAinterference on the proliferation and apoptosis of lung cancer cell

摘要

Survivin, a member of inhibitor of apoptosis family protein, has become an attractive therapeutic target in cancer due to its selective expression in tumor cells and its important roles for tumor cell viability. The aim of this study was to investigate the effect of the survivin-siRNA plasmid on survivin expression in the human lung cancer cell line A549, and to observe its effect on the apoptosis and proliferation of A549 cells. Human lung cancer cell line A549 was transfected with survivin -targeted small interfering RNA (siRNA). The down-regulation of survivin expression was determined by real-time polymerase chain reaction and Western blot. The proliferation of A549 cells was determined by MTT assay. The apoptosis rate and cell cycle distribution were analyzed by flow cytometry (FCM). Caspase-9 activity was also detected to study the apoptosis of lung cancer cells induced by survivin-siRNA. The sequence-specific siRNA efficiently and specifically down-regulated the expression of survivin at both mRNA and protein levels. Down-regulation of survivin expression dramatically suppressed the proliferation of A549 cells, and arrested the cells at G (1)/G (0) phase. Caspase-9 activity was significantly increased in A549 cells transfected with survivin-siRNA. hi this study, we found that Survivin-specific siRNA.