子宫内膜癌中 TβRⅠ和 TβRⅡ基因甲基化状态

摘要

Objective To investigate the correlation between methylation of transforming growth factorβreceptorⅠ,Ⅱ(TβRⅠ,Ⅱ) gene and pathogenesis, development of endometrial cancer (EC).Methods The modified methylation specific PCR ( MSP) was used to detect the methylation status of TβRⅠ,Ⅱgene promoter in 60 cases of EC tissues and 60 cases of adjacent non-cancerous tissues.The correlation between the methylation of two gene promotors and clinical indexes was analyzed.Results The mathylation rate of TβRⅠin EC tissues and adjacent non-cancerous tissues was 75%(45/60) and 45% ( 27/60 ) , respectively. The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues (χ2 =11.25, P <0.01).The mathylation rate of TβRⅡ in EC tissues and adjacent non-cancerous tissues was 68.3%(41/60) and 36.7%(22/60),respectively.The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues (χ2 =12.06, P <0.01 ) .However there was no correlation between the methylation of two gene promotors and clinical indexes ( P >0.05).Conclusion The methylation of TβRⅠand TβRⅡpromoters may be correlated to the pathogenesis of EC.%目的：探讨TβRⅠ和TβRⅡ基因甲基化与子宫内膜癌发生发展的关系。方法应用改进的甲基化特异性聚合酶链式反应（ MSP）技术检测60例子宫内膜癌组织及60例癌旁正常组织中TβRⅠ、TβRⅡ基因启动子甲基化情况，分析两基因启动子甲基化与各临床指标之间的关系。结果 TβRⅠ在子宫内膜癌组和癌旁正常组的甲基化率分别为75 q．0％（45／60）和45．0％（27／60），子宫内膜癌组的甲基化率显著高于癌旁正常组（χ2＝11．250， P ＝0．001）。 TβRⅡ在子宫内膜癌组和癌旁正常组的甲基化率分别为68．3％（41／60）和36．7％（22／60），子宫内膜癌组的甲基化率显著高于癌旁正常组（χ2＝12．063， P ＝0．000）。两基因启动子甲基化与临床指标差异无统计学意义（P＞0．05）。结论 TβRⅠ和TβRⅡ基因启动子甲基化可能与子宫内膜癌的发生有关。