SIMPLE, EFFICIENT AND COST EFFECTIVE METHOD FOR DETECTION OF METHYLATION STATUS OF GENES FROM LIMITED, ARCHIVED FORMALIN FIXED PARAFFIN EMBEDDED (FFPE) AND FINE NEEDLE ASPIRATE CYTOLOGY (FNAC) SAMPLES

摘要

Our experimental results successfully yielded high amount of methylated DNA and showed promoter methylation amplification using very scanty biopsy sample, other clinical FFPE tissues and FNAC cells. Large no of genes could be studied which otherwise not feasible using conventional method of DNA isolation and bisulphite modification. Our method improves substantially the previously described methods in terms of yield, quality using limited amount of tissue from formalin fixed material, and cytology smears from fine needle aspirates