目的：探讨进展期胃癌Rb蛋白表达与E-cadherin及β-catenin在转移淋巴结癌细胞再表达情况及与临床病理因素的关系。方法免疫组织化学染色方法检测Rb在原发灶和E-cadherin及β-catenin在86例进展期胃癌原发灶及相应淋巴结癌细胞表达情况。结果胃癌原发灶中65．12％出现Rb阳性，46．51％转移淋巴结内癌细胞中出现E-cadherin及β-catenin的再表达阳性，Rb与肿瘤的分化程度、病理类型密切相关（ P ＜0．05），E-cadherin及β-cate-nin在转移淋巴结内的再表达阳性与肿瘤的分化程度密切相关（ P ＜0．05），E-cadherin在转移淋巴结内的再表达与肿瘤的病理类型密切相关（ P ＜0．05），β-catenin在转移淋巴结内的再表达与肿瘤的病理类型无关（ P ＞0．05），Rb的表达与E-cadherin及β-catenin在转移淋巴结内癌细胞中的再表达情况、肿瘤的大小及浸润程度无关（ P ＞0．05）。 E-cadherin与β-catenin在转移淋巴结内再表达存在相关关系（ P ＜0．05），Rb在肿瘤原发灶表达与E-cadherin及β-cate-nin在转移淋巴结癌细胞再表达无相关关系（ P ＞0．05）。结论 Rb的表达与进展期胃癌的发展过程密切相关，E-cadherin及β-catenin在转移淋巴结癌细胞的再表达是进展期胃癌发生的重要事件，二者协同发挥作用，并对癌细胞在转移灶中的聚集起重要作用，联合评价其功能具有重要临床意义。%Objective To investigate the expression and clinical significance of Rb protein as well as the re-expression of E-cadherin and β-catenin in cancerous cells of metastatic lymph nodes from advanced gastric carcinoma. Methods The expression levels of Rb protein in primary focus, and expression levels of E-cadherin and β-catenin were detected by immunohistochemistry in primary focus of 86 cases of advanced gastric carcinoma and the corresponding metastatic lymph nodes.Results In primary focus of gastric cancer, the positive rate of Rb protein was 65.12%, and the positive rate of re-expression of E-cadherin andβ-catenin was 46.51%in cancerous cells of metastatic lymph nodes from advanced gastric carcinoma,moreover, the expressions of Rb were closely correlated to the differentiation degree and pathological types of tumor ( P <0.05) .The re-expression levels of E-cadherin and β-catenin in metastatic lymph nodes were closely related with the differentiation degree of tumor ( P <0.05).The re-expression levels of E-cadherin in metastatic lymph nodes were closely correlated to the pathological types of tumor ( P <0.05), however, the re-expression levels ofβ-catenin were not correlated to the pathological types of tumor ( P >0.05).The re-expression levels of Rb protein were not correlated to those of E-cadherin andβ-catenin in cancerous cells of metastatic lymph nodes,and to tumor’s size and infiltration degree ( P >0.05). The re-expression of Ec-adherin was closely correlated to that of βc-atenin in metastatic lymph nodes ( P <0.05),however, the expression levels of Rb protein in primary focus of tumor were not related with those of E -cadherin or βc-atenin in metastatic lymph nodes ( P >0.05) .Conclusion The expression of Rb protein is closely correlated to the pathogenesis and development of advanced gastric carcinoma.The ree-xpressions of E-cadherin and β-catenin in cancerous cells of metastatic lymph nodes from advanced gastric carcinoma play an important role in the pathogenesis of advanced gastric carcinoma ,with a synergism effect,thus, combination detection of E-cadherin with β-catenin has important clinical significance in evaluating their effects in tumor metastasis.
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