目的：探讨NF-κB、Rb蛋白与E-cadherin/catenin复合物对胃癌转移机制和对患者预后的影响。方法采用免疫组化（ SP）法分别检测了NF-κB、Rb蛋白在肿瘤原发灶及E-cadherin/catenin复合物在胃癌原发灶及其相应淋巴结内的表达情况。结果 NF-κB蛋白在胃癌组织中表达阳性率为81．40％，Rb蛋白在胃癌组织中表达阳性率为65．12％， E-cadherin/catenin复合物在胃癌原发灶中异常表达率为98．84％，在转移淋巴结中再表达阳性率为63．95％。 NF-κB表达与胃癌病理类型和分化程度密切相关（P＜0．05）。 Rb蛋白表达与肿瘤分化程度密切相关（P＜0．05）。 E-cadherin/catenin复合物在转移淋巴结中的表达与肿瘤细胞的分化程度密切相关（ P＜0．05）。 Kaplan-Meier曲线显示NF-κB、Rb蛋白的表达与患者的预后无关（P＞0．05）；E-cadherin/catenin复合物在转移淋巴结内表达情况与患者的预后密切相关（P＜0．05）。 Wilcoxon’s log-rank test进行组间差别检验显示各组间差别无显著性（P＞0．05）。 Cox’s proportional-hazard多变量分析显示E-cadherin/catenin复合物在转移淋巴结内的表达可以作为一个独立的预后因素（P＜0．05）。 Sperman秩相关分析显示NF-κB、Rb蛋白表达与E-cadherin/catenin复合物在转移淋巴结内表达均无显著相关性。结论胃癌中存在NF-κB、Rb蛋白的高表达，转移淋巴结中存在E-cadherin/catenin复合物的高表达；E-cadherin/catenin复合物在转移淋巴结内的表达情况与患者的预后密切相关，可以作为一个独立的预后因素。%Objective To study the expression of NF-κB,Rb and the re-expression of E-cadherin/catenin complex in metastatic mechanism of gastric carcinoma and prognosis .Methods S-P immunohistochemical technique was used to examine the expression of NF-κB and Rb in primary advanced gastric carcinoma and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes .Results The positive expression rate of NF-κB was 81.40%in primary advanced gas-tric carcinoma.The positive expression rate of Rb was 65.12%in primary advanced gastric carcinoma .The abnormal expression rate of E-cadherin/catenin complex in primary lesion of gastric cancer was 98.84%,and the positive re-expression rate of E-cad-herin/catenin complex was 63.95%in cancerous cells of metastatic lymph nodes of advanced gastric carcinoma .The expression of NF-κB was closely related to pathological differentiation and tumor pathological stages (P<0.05).The expression of Rb was closely related to pathological differentiation (P<0.05).The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was closely related to pathological differentiation (P<0.05).There is no remarkable difference between the groups by Wilcoxon’s log-rank test.And the Cox’s proportional-hazard showed that the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was a independent factor of prognosis .The expression of NF-κB、Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not significantly correlated ( P>0.05).Conclusion The expression of NF-κB、Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes increased in advanced gastric carcinoma .The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes are closely correlated and could be an independent factor of prognosis .
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