大黄酚对脑缺血再灌注小鼠肺组织过氧化氢和过氧化氢酶的影响

摘要

Objective To study the effects of chrysophanol on hydrogen peroxide and catalase in lung injury induced by cerebral ischemia reperfusion in mice and further analyze the related mechanisms of protective effect on lung injury induced by cerebral ischemia reperfusion .Methods Cerebral ischemia reperfusion models of mice were prepared with 90 Kunming mice anesthetized by 3.5%chloral hydrate .The models of 74 mice that prepared success-fully were randomly divided into five groups:sham-operated group (14 mice, operate but not do cerebral ischemia reperfusion ), model group(IR, 15 mice, operate and do cerebral ischemia reperfusion ), high dose chrysophanol group(IR+Chry, 15 mice, ip 10 mg/kg), middle dose chrysophanol group(IR+Chry, 15 mice, ip 1 mg/kg), low dose chrysophanol group(IR+Chry, 15 mice, ip 0.1mg/kg).Mice of model group and chrysophanol group were be-headed quickly when blood flow is restored after ten minutes , and then lung tissues were taken out of beheaded mice to make 10%lung homogenate .H2 O2 and CAT activities in lung were measured .Results Compared with sham-op-erated group, the content of H2 O2 of model group was increased , and the activities of CAT was decreased , and there were statistical differences ﹙P <0.05);Compared with model group, Chrysophanol could decrease the content of H2O2(Chry10.0, Chry1.0 mg/kg,P<0.01)and increase the activities of CAT(Chry10.0 mg/kg,P<0.01) in lung of mice induced by cerebral ischemia reperfusion .Conclusion Protective effects of chrysophanol on lung injury of cerebral ischemia reperfusion can be affected by increasing the activity of CAT in the pulmonary tissue .%目的：研究大黄酚（ chrysophanol ， Chry ）对脑缺血再灌注（ IR ）后小鼠肺组织中过氧化氢（ H2 O2）及过氧化氢酶（ CAT）的影响，探讨大黄酚对脑IR后对肺损伤的保护作用机制。方法取昆明种小鼠80只，3．5％水合氯醛麻醉后，制备小鼠IR模型。将造模成功的74只小鼠随机分为5组，药物组（ IR＋Chry）45只，分为高、中、低剂量3个组，各15只，模型组15只，假手术组14只。药物组于缺血前30 min 分别腹腔注射大黄酚10．0、1．0、0．1 mg/kg；模型组于缺血前30 min 腹腔注射溶剂10 mL/kg；假手术组只进行手术，但不进行脑IR，腹腔注射溶剂10 mL/kg。模型组和药物组小鼠恢复血供后10 min快速断头处死，取肺组织，制成10％肺组织匀浆，测定小鼠肺组织中H2 O2含量和CAT活力。结果与假手术组比较，模型组小鼠肺组织内H2O2含量明显增加（P＜0．05），肺组织内CAT活力明显降低﹙P＜0．05）；与模型组比较，高剂量大黄酚组小鼠肺组织内H2O2含量明显减少（P＜0．01）。与模型组比较，高剂量大黄酚组小鼠肺组织内CAT活力明显提高（P＜0．01）。结论大黄酚对脑IR肺损伤的保护作用可通过提高肺组织中CAT活力起作用。