Galectin-1、P21、P27在子宫颈鳞状细胞癌和癌前病变中的表达及意义

摘要

目的 分析Galectin-1、P21、P27在子宫颈癌前病变和子宫颈鳞状细胞癌(Cervical squamous cell carcinoma,CSCC)中的表达,探讨其在宫颈癌变过程中的作用机制.方法 采用免疫组织化学S-P法,检测20例正常宫颈组织、39例子宫颈上皮内瘤变(Cervical intraepithelial neoplasia,CIN)Ⅰ、40例CINⅡ、40例CINⅢ和54例CSCC组织中Galectin-1、P21、P27蛋白的表达.结果 Galectin-1和P21在CIN Ⅰ、Ⅱ、Ⅲ和CSCC组织中阳性表达率明显高于正常宫颈组织,差异有统计学意义(P<0.05).P27在CIN Ⅲ和CSCC组织中阳性表达率明显低于正常宫颈组织,差异有统计学意义(P<0.05).但Galectin-1、P21、P27在CIN组间及CSCC组间差异无统计学意义(P>0.05).Galectin-1阳性表达的41例CSCC中P21阳性表达38例,两组间差异有统计学意义(P<0.05).Galectin-1阳性表达的41例CSCC中P27阳性表达13例,两组间差异无统计学意义(P>0.05).结论 Galectin-1 和P21高表达、P27低表达与宫颈癌的发病密切相关,可为宫颈癌前病变发展为浸润性鳞癌提供动态观察的参考指标.%Objective To investigate the expression of Galectin-1, P21 and p27 in cervical squamous cell carcinoma (CSCC) and precancerous change and study the mechanism of carcinogenesis. Methods Using S-P immunohistochemical technique, we examined the expression of Galectin-1, P21 and P27 in 20 cases of normal cervical tissues,39 cases of cervical intraepithelial neoplasm (CIN) Ⅰ ,40 cases of CIN Ⅱ, 40 cases ofCIN Ⅲ and 54 cases of CSCC.Results Expression of Galectin-1 and p27 positive in CIN Ⅰ, CIN Ⅱ, CIN Ⅲ and CSCC were higher markedly than that in normal cervical tissues, with the significant difference (P＜0.05). Expression of p27 positive in CIN Ⅰ, CIN Ⅱ,CIN Ⅲ and CSCC were lower markedly than that in normal cervical tissues, with the significant difference (P＜0.05).But the expression of Galectin-1, P21 and p27in groups of CSCC and CIN were not difference. Expression of Galectin-1 positive in 41 cases CSCC which was P27positive in 38 cases with it, and the difference was significant (P＜0.05).Expression of Galectin-1 positive in 41 CSCC which was p27 positive in 13 cases with it, the difference was not significant. Conclusion The higher expression of Galectin-1 and P21and the lower expression of P27 were relational in CSCC invasion, and they will become the target to observe the evolution from precancerous change to CSCC.