活化的淋巴细胞对人骨髓间质干细胞的细胞毒性研究

摘要

目的 利用体外混合培养方法深入研究间质干细胞和免疫细胞的相互作用,为间质干细胞的合理应用提供理论依据.方法 分别利用贴壁培养和密度梯度离心的方法获得人骨髓间质干细胞和外周血单核细胞,利用体外混合培养的方法研究间质干细胞和淋巴细胞的相互作用.结果 人骨髓来源的间质干细胞能够抑制淋巴细胞的增殖,但是也很容易遭到活化淋巴细胞的毒性攻击.活化淋巴细胞分泌的炎症因子能够驱使淋巴细胞向间质干细胞周围移动从而加强彼此间的相互作用.作为一种反应机制,间质干细胞在炎症因子刺激后能够获得一定程度上的对活化淋巴细胞的细胞毒性抗性.间质干细胞最终能否发挥免疫调节作用将取决于双方力量的平衡.结论 体外研究结果提示人骨髓间质干细胞对活化淋巴细胞的细胞毒性敏感,该结果可能反映了间质干细胞输入体内后的情况,为间质干细胞的临床应用提供了新的思考和借鉴.%Objective To investigate the reciprocal interaction between mesenchymal stem cells (MSCs) and immune cells, and to provide a reference for the application of mesenchymal stem cells. Methods MSCs and peripheral blood mononuclear cells were collected by adherent culture and density gradient centrifugation. Then mixed culture was performed to investigate the interaction between MSCs and immune cells. Results MSCs were prone to be attacked by activated lymphocytes, though they also inhibited the proliferation of lymphocytes. Lymphocytes could converge to the vicinity of hMSCs after hMSCs were stimulated by supernatant media derived from activated lympho-cyres. This proximity provide hMSCs and activated lymphocytes the opportunity of close contact and interaction. Correspondingly, hMSCs also acquired some degree of resistance to the attack of activated lymphocytes after they were stimulated by inflammatory factors. Whether hMSCs could ultimately exert immunoregulatory function would depend on mutual competition of hMSCs and immune cells. Conclusion MSCc are sensitive to the cytotoxicity of activated lymphocytes. This study describes what's happening after hMSCs are infused into body, which provides some consideration during the clinical application of hMSCs.