目的 探讨抑凋亡蛋白Survivin及多药耐药基因mdr1表达的P-糖蛋白(P-gp)的表达与骨肉瘤临床病理特征间的关系.方法 应用免疫组织化学技术(SP法)检测34例骨肉瘤组织及30例骨软骨瘤(对照组)组纲中抑凋亡蛋白Survivin及多药耐药基因mdr1表达的P-糖蛋白的表达,统计分析Survivin、P-gp在骨肉瘤组织中表达的相关性和其表达情况与病例临床特征的相关性.结果 ①34例骨肉瘤组织标本中19例Survivin蛋白表达阳性为55.9％ (19/34),27例P-gp表达阳性为79.4％ (27/34);对照组30例标本中未检测到Survivin蛋白表达、P-gp 表达(P＜0.01).②39例骨肉瘤患者中发生转移的病例16例,其Survivin蛋白、P-gp表达水平显著高于未发生转移病例(P＜0.05).结论 抑凋亡蛋白Survivin及多药耐药基因mdr1表达的P-gp的在骨肉瘤组织中呈现高表达,且两者表达水平与骨肉瘤病理分级和转移成正比.%Objective To investigate the expression of Survivin and P-glycoprotein (P-gp) in osteosarcoma and its relationship with the clinical and pathological features of osteosarcoma. Methods The expression of antiapop-totic protein Survivin and P-gp (expressed by multidrug resistance gene mdrl) were detected in 34 cases of osteosarco-ma (the study group) and 30 cases of osteochondroma (the control group) using immunohistochemistry (SP method). The relationship between the expression of Survivin and P-gp, as well as its relevance to the clinical and pathological features of osteosarcoma was analyzed. Results ① The positive expression of Survivin protein was found in 19 of the 34 cases of osteosarcoma, accounting for 55.9% (19/34), and the positive expression of P-gp was found in 27 cases (79.4%, 27/34). Specimens in the control group were found with no expression of Survivin and P-gp protein. ② 16 cases in the study group were found with osteosarcoma metastasis, in which the expression of Survivin and P-gp pro-tein was significantly higher than those patients without metastasis (P <0.05). Conclusion Survivin and P-gp show high expression in osteosarcoma, and the expression levels of the two proteins is positively related to tumor grade and metastasis of osteosarcoma.
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