目的：研究不同程度积水肾组织中E-cadherin mRNA基因及蛋白表达。方法50例患者，年龄18~75岁，通过肾脏彩超将其分为轻度、中度、重度肾积水，每组分别为15例、18例、17例，同时选取健康体检正常人群50例作为对照组；肾脏穿刺取肾皮质组织，Realtime RT-PCR及Western blot分别检测各组E-cadherin mRNA基因及蛋白表达；分析E-cadherin表达与肾积水程度之间的关系。结果正常对照组肾脏表达非常丰富的E-cadherin mRNA基因及蛋白，而积水肾组织中E-cadherin mRNA基因及蛋白表达均显著下降；同时随着积水程度的不断加重，E-cadherin表达水平进行性下降，并与肾积水程度之间存在明显负相关关系。结论肾积水将通过不同机制下调E-cadherin表达，并进而通过EMT等中间环节导致肾小管间质纤维化，E-cadherin在其中起着非常重要的作用。%Objective To investigate the expression of E-cadherin in different levels of hydronephrosis and investigate its clinical significance. Methods Fifty patients were enrolled in the study, with age of 18~75 years old. They were divided into mild, moderate and severe hydronephrosis groups, with 15, 18 and 17 patients respectively. Fifty healthy people were selected as control group. Real time PCR and Western blot were used to detect the expression of E-cadherin mRNA and protein. Correlation analysis was applied to find out their relationships. Results E-cadherin was highly ex-pressed in the control group, while its expression was low in the hydronephrosis groups. And the more severe the hydrone-phrosis was, the lower the E-cadherin expression level. The expression of E-cadherin was negatively correlated with the se-verity of hydronephrosis. Conclusion The hydronephrosis would cause the downregulation of E-cadherin expres-sion through many different mechanisms, and would cause tubulointerstitial fibrosis through EMT ultimately. E-cad-herin playes very important roles during the process.
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