参松养心胶囊对心梗后心肌的保护作用及机制研究

摘要

Objective To explore the effect of shensongyangxin capsule (SSYX) on arrhythmia and cardiac insufficiency after myocardial infarction (MI) in rabbits.Methods Forty New Zealand White rabbits were ran-domly assigned into control group,MI group,MI+amiodarone group (MI+A group,with the dose of amiodarone at 0.4 g·kg-1·d-1) and MI+SSYX group (MI+S group,with the dose of SSYX at 0.5 g·kg-1·d-1) after successful establish-ment of model of MI,each with 10 rabbits.The control group applied stitches at coronary artery without ligation.The other three groups applied routine food,routine food+amiodarone,routine food+SSYX after surgery,respectively.After eight weeks,ryanodine receptor 2 (RyR2),Connexin43 (CX43),sodium-calcium exchanger (NCX) mRNA expression in peri-infarcted area were examined.Results Compared with control group,CX43,RyR2 mRNA relative expression sig-nificantly decreased in MI group (P<0.05),but NCX mRNA relative expression showed slight decrease without statisti-cally significant difference (P>0.05).Compared with MI group,CX43,RyR2 mRNA relative expression significantly in-creased in MI+A group (both P<0.05),and CX43,NCX,RyR2 mRNA relative expression significantly increased in MI+S group (all P<0.05).Compared with MI+A group,CX43,NCX,mRNA relative expression significantly increased in MI+S group (all P<0.05).Conclusion SSYX can improve arrhythmia and cardiac dysfunction after myocardial infarc-tion,which may be related to the open of calcium ion channels and the inhibition of calcium overload.%目的 探讨参松养心胶囊(SSYX)对心肌梗死后心律失常发生和心功能不全的影响及其作用机制.方法 将40只新西兰大耳白兔行冠状动脉前降支结扎术后随机分为正常对照组(Control组) 10只、心梗组(MI组)10只、心梗+胺碘酮组(MI+A组,0.4 g·kg-1·d-1) 10只、心梗+参松组(MI+S组,0.5 g·kg-1·d-1) 10只.正常对照组开胸后在冠状动脉下穿线,但不结扎.术后,分别以普食、普食+胺碘酮粉末、普食+参松养心胶囊原粉喂食.8周后将家兔处死,在心梗周边区取材,检测各组白兔缝隙连接蛋白43 (CX43)、钠钙交换体(NCX)和兰尼碱受体蛋白(RyR2) mRNA的相对表达量.结果 与Control组比较,MI组CX43、RyR2 mRNA相对表达量显著下降(P<0.05),而NCX mRNA相对表达量虽然有所下降,但其差异无统计学意义(P>0.05);与MI组比较,MI+A组CX43、RyR2 mRNA相对表达量显著升高(P<0.05),MI+S组CX43、NCX、RyR2 mRNA的相对表达量也显著升高(P<0.05);与MI+A组比较,MI+S组CX43、NCX mRNA的相对表达量均显著升高(P<0.05).结论 参松养心胶囊可以改善心肌梗死后心律失常以及心功能不全,其机制可能与调控各钙离子通道的开放,抑制钙超载有关.